What effect did boiling have on enzyme activity Why How well did the results compare with your prediction?
How well did the results this compare with your prediction? Your answer: Boiling altered the enzyme and deactivated the amylase activity as I predicted.
What effect did boiling and freezing have on enzyme activity Why How well did the results compare with your prediction quizlet?
What effect do you think boiling and freezing will have on the activity of the amylase enzyme? Boiling will decrease amylase activity and freezing will have no effect.
What substance is being liberated to affect the pH in tubes 1 and 2?
What substance is being liberated to affect the pH in tubes 1 and 2? Fatty acids are released when triglycerides are broken down with lipase. The release of the fatty acids causes the pH to decrease, making it more acidic.
Which tube has the highest lipase activity How well did the results?
The correct prediction is tube #1, pH 7.0, which approximates the pH of the small intestine. Since the activity of pancreatic lipase is highest at pH 7.0, the enzyme should be active in the mouth and the pancreas.
Which two tubes validate the results of the experiment?
Which two tubes validated the results of the experiment? You correctly answered: tubes 3 (pepsin, deionized water, pH 2 buffer) and 4 (deionized water, BAPNA, pH 2 buffer).
What is the effect of boiling the enzyme in tube 1 first before incubation?
Boiling ended the enzyme activity (as seen by test tube 1 when it was boiled before being incubated) because the head caused the reactants and enzyme to denature and stop.
What effect did freezing have on enzyme activity Why?
Effect of Freezing on Enzyme Activity At very cold temperatures, the opposite effect dominates – molecules move more slowly, reducing the frequency of enzyme-substrate collisions and therefore decreasing enzyme activity.
Which tube allows you to conclude that the buffer?
3. Which tube allows you to conclude that the buffer was not contaminated with amylase? You correctly answered: tube 5 (peptidase, starch, pH 7 buffer).
Which tube confirms that there is no lipase in?
tube 4 Which tube confirms that there is no lipase in bile salts or vegetable oil? You correctly answered: tube 4 (deionized water, vegetable oil, bile salts, pH 7.0 buffer).
Which two tubes validated the results of the protein digestion exercise?
Which two tubes validated the results of the experiment? You correctly answered: tubes 3 (pepsin, deionized water, pH 2 buffer) and 4 (deionized water, BAPNA, pH 2 buffer).
How do you validate an experiment?
To carry out a good method validation study, you need to do the following:
- define a quality requirement for the test in the form of the amount of error that is allowable, preferably an allowable total error,
- select appropriate experiments to reveal the expected types of analytical errors,
Why was there a difference in optical densities between tubes 1 & 2?
Tube 1 and 2 different in their optical density measurements because tube 1 was boiled first and then incubated. By boiling first, the enzyme pepsin was denatured because the temperature was too high resulting in an optical density of 0.00 (there was no digestion of BAPNA by pepsin).
Why changing the shape of an enzyme could affect the ability of the enzyme to function?
If the enzyme changes shape, the active site may no longer bind to the appropriate substrate and the rate of reaction will decrease. Dramatic changes to the temperature and pH will eventually cause enzymes to denature.
Why do enzymes slow down in cold temperatures?
Lowering the temperature slows the motion of molecules and atoms, meaning this flexibility is reduced or lost. Each enzyme has its zone of comfort, or optimal temperature range, within which it works best. As the temperature decreases, so does enzyme activity.
Which tube is included to detect contaminating amylase in the buffer or in cellulose?
Which tube is included to detect contaminating amylase in the buffer or in cellulose? Your answer: tube 4 (cellulose, pH 7 buffer, deionized water).
Which tube had the highest lipase activity How well did the results compare with your prediction discuss the possible reasons why it may or may not have matched?
After completing the experiment it was identified that the tube with the highest lipase activity would be tube 1, this is because it has the closet pH to the intestines.
What is the purpose for including tube 3 and what can you conclude from the result?
What is the purpose for including tube 3 and what can you conclude from the result? To see the comparison between boiling or freezing before incubation and straight incubation with pH 7.0 buffer, 70oC, for 60minutes.
How the results of the experimental data are validated?
You can use response surface methodology (RSM) or Principle Component Analysis (PCA) for validation and optimisation of you results.
What is knowledge validation in expert system?
Validation is a critical process in the whole knowledge-based system life cycle. A knowledge base incorporated into such systems has to be verified or (more generally) validated. There have been many approaches to develop specialised procedures and techniques, aimed at assuring the highest level of knowledge quality.
What is difference between optical density and absorbance?
Difference between Optical Density and Absorbance Optical density measures the degree of the angle to which a particular medium slows down the speed of the transmitted light. Absorbance measures the capacity of a particular object or a medium to absorb the transmitted light that is of a specific wavelength.
What factors affect enzyme activity explain each of the factors?
Factors affecting enzyme activity Enzyme activity can be affected by a variety of factors, such as temperature, pH, and concentration. Enzymes work best within specific temperature and pH ranges, and sub-optimal conditions can cause an enzyme to lose its ability to bind to a substrate.
What determines the shape of an enzyme in what ways may an enzyme’s shape be altered Why might this cause malfunction?
Proteins change shape as temperatures change. Because so much of an enzyme's activity is based on its shape, temperature changes can mess up the process and the enzyme won't work. High enough temperatures will cause the enzyme to denature and have its structure start to break up.
How does temperature change affect enzyme activity?
An increase in temperature generally increases the enzyme activity and rate of reaction and lowering the temperature slows down the enzymatic reaction. Each enzyme shows maximum activity at the specific optimum temperature and activity declines above and below the optimum temperature.
Does the same thing happen when enzymes get too hot Why or why not?
Does the same thing happen to enzymes when they get too hot and when they get too cold? why or why not? No, they enzymes will change shape or denature causing them not to work at all when they get too hot.
Which of the following explains why each step in the metabolic pathway often requires a separate enzyme?
Which of the following explains why each step in the metabolic pathway often requires a separate enzyme? Enzymes are specific for their substrate. The following shows a metabolic pathway: What would be the effect on the reaction of adding a competitive inhibitor to enzyme 2?
Why does a positive Benedict test result confirm?
Why does a positive Benedict test result confirm that the unknown solution originally contained starch? A positive result indicates simple sugars (maltose) that result from starch being digested by amylase.
How do you validate scientific research?
Compare with previous research studies or benchmark. Whenever, write a review a paper or edit a paper for peer review journal, a key item that we should look for is whether the methodology and equipment has been validated, demonstrating the effectiveness (accuracy and reliability) of the research.
What is validation in an experiment?
Validation experiments define procedural limitations, identify critical components of the procedure that require quality control and monitoring, and establish standard operating procedures and interpretation guidelines for laboratories to follow while processing samples.
How do you validate information and knowledge?
To validate any data received from others, make sure beforehand that you were clear as to what information you were asking for. It is a good idea to give an explanation, and then follow through with an example for further clarification of what material you were seeking.
What is knowledge validation process?
Validation is a critical process in the whole knowledge-based system life cycle. A knowledge base incorporated into such systems has to be verified or (more generally) validated. There have been many approaches to develop specialised procedures and techniques, aimed at assuring the highest level of knowledge quality.