Can Benedict’s solution distinguish between glucose and fructose?
– However, Benedict's solution cannot be used to distinguish between glucose and fructose because it is used to differentiate between reducing and non-reducing sugars, and Fructose and glucose are both reducing sugars. So, the correct answer is “Option C”.
Why does the Benedict’s reagent react with glucose and fructose but not sucrose?
Sucrose contains two sugars (fructose and glucose) joined by their glycosidic bond in such a way as to prevent the glucose isomerizing to aldehyde, or the fructose to α-hydroxy-ketone form. Sucrose is thus a non-reducing sugar, which does not react with Benedict's reagent.
Does Benedict’s solution detect fructose?
The products of sucrose decomposition are glucose and fructose, both of which can be detected by Benedict's reagent as described above.
Why is Benedict not exclusive to glucose?
The Benedict test isn't specific for glucose; it just tells you if an aldehyde is present. So it will also give a positive test for other reducing sugars.
How will you distinguish between glucose and fructose?
Example of monosaccharides – glucose and fructose….Fructose.
| Difference between Glucose and Fructose | |
|---|---|
| GLUCOSE | FRUCTOSE |
| Relies on hexokinase/ glucokinase for initiating metabolism | Relies on fructokinase for initiating metabolism |
| It is an aldohexose | It is a ketohexose |
| Forms a pyranose ring structure | Forms a furan ring structure |
Which test is used to distinguish between glucose and fructose?
(d) Seliwanoff's Test: It is used to distinguish between the aldose and ketose. So, from this it is clear that we can distinguish between the fructose and glucose by Seliwanoff's test because fructose is a ketose and glucose is an aldose.
Why is fructose positive in Benedict’s test?
Thus, although the ketose fructose is not strictly a reducing sugar, it is an alpha-hydroxy-ketone, and gives a positive test because it is converted to the aldoses glucose and mannose by the base in the reagent.. Benedict's reagent can be prepared from sodium carbonate, sodium citrate and copper(II) sulfate.
Which reagent can distinguish between glucose and fructose?
> Tollens reagent gives positive tests with aldehydes and alpha hydroxy ketones. Glucose being an aldehyde gives positive test with Tollen's reagent. Fructose being alpha hydroxy ketone gives positive test with Tollen's reagent.
Which test can distinguish between glucose and fructose?
(d) Seliwanoff's Test: It is used to distinguish between the aldose and ketose. So, from this it is clear that we can distinguish between the fructose and glucose by Seliwanoff's test because fructose is a ketose and glucose is an aldose.
How can fructose be distinguished from glucose?
What are Some of the Common Differences Between Glucose and Fructose? Ans: Glucose is a 6 membered ring, whereas fructose is a 5 membered ring. Glucose produces less fat compared to fructose in our bodies. Glucose is aldohexose, whereas Fructose is Ketohexose.
Which does not give Benedict’s test?
The correct option is D i.e. sucrose.
Which sugar does not give Benedict’s test?
Sucrose Sucrose is thus a non-reducing sugar, which does not react with Benedict's reagent.
Which test should be used to best identify the presence of fructose?
Most commonly used tests for detection of reducing sugars are Fehling's Test, Benedict's Test and Barfoed's Test. a) Fehling's Test: Fehling's Solution (deep blue colored) is used to determine the presence of reducing sugars and aldehydes. Perform this test with fructose, glucose, maltose and sucrose.
Why is Seliwanoff’s test is said to distinguish between fructose and glucose?
The high concentration of glucose or other sugar may interfere by producing similar colored compounds with Seliwanoff's reagent. Prolonged boiling can transform glucose to fructose by the catalytic action of acid and form cherry red-complex giving a false-positive result.
How does Benedict’s solution detect glucose?
In lab, we used Benedict's reagent to test for one particular reducing sugar: glucose. Benedict's reagent starts out aqua-blue. As it is heated in the presence of reducing sugars, it turns yellow to orange. The "hotter" the final color of the reagent, the higher the concentration of reducing sugar.
How do you separate glucose and fructose?
Fructose from type A ionic liquid or glucose from type B ionic liquid with a purity of more than 99% can be separated from the saturated ionic liquid using filtration or centrifuging.
Why doesn’t the Benedict’s test work for all sugars?
Complex carbohydrates such as starches DO NOT react positive with the Benedict's test unless they are broken down through heating or digestion (try chewing crackers and then doing the test). Table sugar (disaccharide) is a non-reducing sugar and does also not react with the iodine or with the Benedict Reagent.
Which sugar does not give Benedict test?
Sucrose is thus a non-reducing sugar, which does not react with Benedict's reagent.
How can we separate glucose and fructose?
Fructose from type A ionic liquid or glucose from type B ionic liquid with a purity of more than 99% can be separated from the saturated ionic liquid using filtration or centrifuging.
What is in Benedict’s solution?
Benedict's reagent, also known as Benedict's solution, is a chemical reagent which is made up of a complex mixture of sodium citrate, sodium carbonate, and the pentahydrate of copper(II) sulfate.
What is the difference between glucose and fructose test?
The glycemic index is lower in fructose when compared to glucose. The binding of fructose to cellular protein is seven times faster than glucose. Fructose is also called as fruit sugar or D- fructose. Its functional group is a ketone.
What is the difference between the glucose ring and the fructose ring in the sucrose molecule?
Glucose is six membered ring, while fructose is a five-membered ring. Both rings contain an oxygen atom.
Can glucose give Benedict test?
It can be noted that Benedict's test can also be used to check for the presence of glucose in a urine sample. Since this test detects any aldehydes and α-hydroxy ketones and glucose is an aldose whose open-chain forms an aldehyde group, the test yields a positive result when glucose is present in the analyte.
What test distinguish glucose from fructose?
(d) Seliwanoff's Test: It is used to distinguish between the aldose and ketose. So, from this it is clear that we can distinguish between the fructose and glucose by Seliwanoff's test because fructose is a ketose and glucose is an aldose.
What are the limitations of the Benedict’s test?
Benedict's test has the following limitations: Penicillin, isoniazid, streptomycin, salicylates, and p-aminosalicylic acid have all produced false-positive results. Chemicals such as creatinine, ascorbic acid, and urate slow Benedict's reaction in the urine.
How can we distinguish between glucose and fructose?
Glucose and fructose are simple sugars. Simple carbohydrates are classified into two types. They are monosaccharides and disaccharides….Fructose.
| Difference between Glucose and Fructose | |
|---|---|
| GLUCOSE | FRUCTOSE |
| It is an aldohexose | It is a ketohexose |
| Forms a pyranose ring structure | Forms a furan ring structure |
Can glucose be separated?
Glucose can be separated by using different columns with size exclusion or amino sorbents (25, 26), even with a low temperature ELSD (ELSD-LT) (27).
How do you separate glucose from a mixture of glucose and maltose?
Answer. Answer: nanofiltration is a method used to separate glucose from a mixture of glucose and maltose ….
Why does glucose react with Benedict’s solution?
Some sugars such as glucose are called reducing sugars because they are capable of transferring hydrogens (electrons) to other compounds, a process called reduction. When reducing sugars are mixed with Benedicts reagent and heated, a reduction reaction causes the Benedicts reagent to change color.
Which of the following is used to distinguish between glucose and fructose?
Solution : Glucose as well as fructose give silver mirror test with Tollen. s reagent and red ppt of `Cu_2O` with Fehling solution and Benedict. s solution. Step by step solution by experts to help you in doubt clearance & scoring excellent marks in exams.