What is the correct order for Gram staining?
The stains are applied to a smear of bacteria on a microscope slide in the following order: crystal violet, Gram's iodine, decolorizing agent, and safranin.
What is the correct order for the Gram stain quizlet?
Terms in this set (28) Place the steps of the Gram stain in the correct order:1-Alcohol-acetone; 2-Crystal violet; 3-Safranin; 4-Iodine.
What are the steps in a Gram stain and the possible results?
A Gram stain is colored purple. When the stain combines with bacteria in a sample, the bacteria will either stay purple or turn pink or red. If the bacteria stays purple, they are Gram-positive. If the bacteria turns pink or red, they are Gram-negative.
What are the steps of the Gram stain quizlet?
Steps of gram staining technique:
- Apply primary stain (crystal violet). All bacteria are stained purple by this basic dye.
- Apply mordant (Gram's iodine). …
- Apply decolorizing agent (ethyl alcohol or ethyl alcohol acetone). …
- Apply secondary stain or counterstain (safranin).
What is the correct order of staining reagents in Gram staining Mcq?
Explanation: Gram staining is a type of differential staining. In this process the fixed bacterial smear is subjected to the following staining reagents in the order listed: crystal violet, iodine solution, alcohol (decolorizing agent), and safranin.
What is the Gram stain method quizlet Labster?
gram stain. a differential staininging technique that differentiates nearly all bacteria species into two large groups based on structural differences in the cell wall.
What is the first step in the gram staining process?
The first step in gram staining is the use of crystal violet dye for the slide's initial staining. The next step, also known as fixing the dye, involves using iodine to form crystal violet- iodine complex to prevent easy removal of dye.
Which of the following is the correct order of the Gram stain reagents quizlet?
List the 4 reagents used for the gram stain in the proper order? Crystal violet stains gram positive and gram negative cells. Iodine is used next as mordant. Alcohol decolorizes the gram negative cells, and lastly Safranin counterstains gram negative cells.
What are the 4 steps of Gram staining?
The performance of the Gram Stain on any sample requires 4 basic steps that include applying a primary stain (crystal violet) to a heat-fixed smear, followed by the addition of a mordant (Gram's Iodine), rapid decolorization with alcohol, acetone, or a mixture of alcohol and acetone and lastly, counterstaining with …
What is the 4/stage process of Gram staining?
The performance of the Gram Stain on any sample requires 4 basic steps that include applying a primary stain (crystal violet) to a heat-fixed smear, followed by the addition of a mordant (Gram's Iodine), rapid decolorization with alcohol, acetone, or a mixture of alcohol and acetone and lastly, counterstaining with …
What is the first step in Gram staining?
The first step in gram staining is the use of crystal violet dye for the slide's initial staining. The next step, also known as fixing the dye, involves using iodine to form crystal violet- iodine complex to prevent easy removal of dye.
What are the four reagents used in the Gram stain?
Reagents needed for Gram staining include:
- Crystal violet (primary stain) (1)
- Gram's iodine solution (the mordant) (1)
- Acetone/ethanol (50:50 v:v) (the decolorizer) (1)
- 0.1% basic fuchsin solution (the counterstain) (1)
- Water.
Aug 11, 2021
Which of the following correctly describe how the Gram stain works?
which of the following correctly describe how the Gram stain works to color different types of cells? gram-positive cells retain the purple dye-mordant complex because of their thick layer of peptidoglycan.
How does the Gram stain work?
The basic principle of gram staining involves the ability of the bacterial cell wall to retain the crystal violet dye during solvent treatment. Gram-positive microorganisms have higher peptidoglycan content, whereas gram-negative organisms have higher lipid content.
What is the third step of Gram staining?
After the rinse, Gram's iodine is added to chemically bond the alkaline dye to the bacterial cell wall, if possible. The third step is the decolorizing step where the slide is exposed to an alcohol wash to take off any unbound crystal violet. The last step is the application of a counterstain.
What 4 reagents are used in the Gram stain?
Reagents needed for Gram staining include: Gram's iodine solution (the mordant) (1) Acetone/ethanol (50:50 v:v) (the decolorizer) (1) 0.1% basic fuchsin solution (the counterstain) (1) Water.
What is Gram staining and explain its procedure?
The procedure is based on the reaction between peptidoglycan in the cell walls of some bacteria. The Gram stain involves staining bacteria, fixing the color with a mordant, decolorizing the cells, and applying a counterstain. The primary stain (crystal violet) binds to peptidoglycan, coloring cells purple.
What are the four stages of Gram staining?
The Gram staining process includes four basic steps, including:
- Applying a primary stain (crystal violet).
- Adding a mordant (Gram's iodine).
- Rapid decolorization with ethanol, acetone or a mixture of both.
- Counterstaining with safranin.
Mar 16, 2022
How do you prepare a Gram stain for a smear?
Preparation of the smear is as follows:
- From broth: Using a cooled, sterile loop, place a loopful of broth on the slide and spread in a circular motion to about 1 cm in diameter.
- From plated media: Place a drop of sterile water or saline on the slide. Select the isolated colony to be stained.
Which of the following correctly describes how the Gram stain works to color?
which of the following correctly describe how the Gram stain works to color different types of cells? gram-positive cells retain the purple dye-mordant complex because of their thick layer of peptidoglycan.
What is the correct sequence for making a specimen smear on a slide?
In order to stain the bacterial specimen for microscopy one must first prepare the smear on the slide. This basically involves three steps—-transferring a liquid suspension of the bacterium on the slide, drying the smear, and then heating slightly to firmly attach the smear to the slide.
What are the 4 steps of gram staining?
The performance of the Gram Stain on any sample requires 4 basic steps that include applying a primary stain (crystal violet) to a heat-fixed smear, followed by the addition of a mordant (Gram's Iodine), rapid decolorization with alcohol, acetone, or a mixture of alcohol and acetone and lastly, counterstaining with …
What is the third step of gram staining?
After the rinse, Gram's iodine is added to chemically bond the alkaline dye to the bacterial cell wall, if possible. The third step is the decolorizing step where the slide is exposed to an alcohol wash to take off any unbound crystal violet. The last step is the application of a counterstain.
What is gram staining and explain its procedure?
The procedure is based on the reaction between peptidoglycan in the cell walls of some bacteria. The Gram stain involves staining bacteria, fixing the color with a mordant, decolorizing the cells, and applying a counterstain. The primary stain (crystal violet) binds to peptidoglycan, coloring cells purple.
What is the correct order of steps in smear preparation?
SMEAR PREPARATION
- Place one needle of solid bacterial growth or two loops. of liquid bacterial growth in the center of a clean slide.
- If working from a solid medium, add one drop (and only one drop) …
- Now, with your inoculating loop, mix the specimen with the water. …
- Place the slide on a slide warmer and wait for it to dry.
What are the steps of simple staining in the correct order?
Simple stains are just that – add one stain to a fixed smear slide, let it sit, rinse it off, let it dry, and view….Simple Stain Procedure
- Do one slide at a time.
- Cover the smear with any of the basic dyes available to you.
- You only need enough dye to cover the smear. The stain should not drip off the slide.
What is the correct order of steps in preparing a bacterial smear slide for staining?
In order to stain the bacterial specimen for microscopy one must first prepare the smear on the slide. This basically involves three steps—-transferring a liquid suspension of the bacterium on the slide, drying the smear, and then heating slightly to firmly attach the smear to the slide.