What is the correct order for Gram staining?
The stains are applied to a smear of bacteria on a microscope slide in the following order: crystal violet, Gram's iodine, decolorizing agent, and safranin.
Which is the correct order of steps in Gram staining quizlet?
In this microscope, the observer does NOT look at an image through a lens. Place the steps of the Gram stain in the correct order: 1-Alcohol-acetone; 2-Crystal violet; 3-Safranin; 4-Iodine.
What are the 5 steps of Gram staining?
There are six basic steps:
- Apply a smear of bacteria on to a slide. …
- Add about 5 drops of Hucker's Crystal Violet to the culture. …
- Add about 5 drops of iodine solution to the culture. …
- Tilt slide and decolorize with solvent (acetone-alcohol solution) until purple color stops running. …
- Add about 5 drops of Safranine O.
What is the first step of Gram staining?
The first step in gram staining is the use of crystal violet dye for the slide's initial staining. The next step, also known as fixing the dye, involves using iodine to form crystal violet- iodine complex to prevent easy removal of dye.
What are the 4 steps of Gram staining?
The performance of the Gram Stain on any sample requires 4 basic steps that include applying a primary stain (crystal violet) to a heat-fixed smear, followed by the addition of a mordant (Gram's Iodine), rapid decolorization with alcohol, acetone, or a mixture of alcohol and acetone and lastly, counterstaining with …
What is the correct order of staining reagents in Gram staining Mcq?
Explanation: Gram staining is a type of differential staining. In this process the fixed bacterial smear is subjected to the following staining reagents in the order listed: crystal violet, iodine solution, alcohol (decolorizing agent), and safranin.
What are the 4 steps of gram staining?
The performance of the Gram Stain on any sample requires 4 basic steps that include applying a primary stain (crystal violet) to a heat-fixed smear, followed by the addition of a mordant (Gram's Iodine), rapid decolorization with alcohol, acetone, or a mixture of alcohol and acetone and lastly, counterstaining with …
What are the steps of simple staining in the correct order quizlet?
- Place a small drop of water on a clean microscope slide.
- Aseptically obtain a small loop of bacteria and mix with water.
- Evenly spread bacteria across slide to make a thin smear.
- Allow slide to air dry.
- Heat fix slide.
- Apply stain to slide for 1 to 2 minutes.
- Rinse stain off slide water.
What is the third step of Gram staining?
After the rinse, Gram's iodine is added to chemically bond the alkaline dye to the bacterial cell wall, if possible. The third step is the decolorizing step where the slide is exposed to an alcohol wash to take off any unbound crystal violet. The last step is the application of a counterstain.
What are the four stages of Gram staining?
The Gram staining process includes four basic steps, including:
- Applying a primary stain (crystal violet).
- Adding a mordant (Gram's iodine).
- Rapid decolorization with ethanol, acetone or a mixture of both.
- Counterstaining with safranin.
Mar 16, 2022
What is the Gram stain method quizlet?
Gram stain technique. A staining procedure used to identify bacterial cells as gram-positive or gram-negative. developed by christian gram in the 1800s. -Cells are stained with crystal violet and Gram iodine solution and washed with a decolorizer.
What are the four reagents used in the Gram stain?
Reagents needed for Gram staining include:
- Crystal violet (primary stain) (1)
- Gram's iodine solution (the mordant) (1)
- Acetone/ethanol (50:50 v:v) (the decolorizer) (1)
- 0.1% basic fuchsin solution (the counterstain) (1)
- Water.
Aug 11, 2021
Which of the following is the correct order of the Gram stain reagents quizlet?
List the 4 reagents used for the gram stain in the proper order? Crystal violet stains gram positive and gram negative cells. Iodine is used next as mordant. Alcohol decolorizes the gram negative cells, and lastly Safranin counterstains gram negative cells.
Which is the correct order for the application of Regents in the Gram stain quizlet?
Terms in this set (28) Place the steps of the Gram stain in the correct order:1-Alcohol-acetone; 2-Crystal violet; 3-Safranin; 4-Iodine.
Which step in the Gram stain is the critical step?
The critical step of the Gram staining procedure is the decolorization step. Hold the slide in a tilted downward position and allow the decolorizer to flow over the smear.
What is the 4/stage process of gram staining?
The performance of the Gram Stain on any sample requires 4 basic steps that include applying a primary stain (crystal violet) to a heat-fixed smear, followed by the addition of a mordant (Gram's Iodine), rapid decolorization with alcohol, acetone, or a mixture of alcohol and acetone and lastly, counterstaining with …
What are the 4 stains used in gram staining?
Crystal violet (primary stain) Iodine solution/Gram's Iodine (mordant that fixes crystal violet to cell wall) Decolorizer (e.g. ethanol) Safranin (secondary stain)
What is the correct ordering to order to stain a microbe?
Place the steps for preparing a simple stain in the correct order: –Evenly spread bacteria across slide to make a thin smear. -Apply stain to slide for 1 to 2 minutes. -Rinse stain off slide water.
Which is the correct order of the reagents used in the acid-fast staining procedure from left to right?
The correct order of an acid-fast stain is primary staining, iodine fixation, decolorizing, counterstaining.
What is the Gram stain method quizlet Labster?
gram stain. a differential staininging technique that differentiates nearly all bacteria species into two large groups based on structural differences in the cell wall.
Which step in the Gram stain is the critical step in differentiating Gram-positive?
decolorization step In addition to the clearly gram-positive and gram-negative, there are many species that are “gram-variable” with intermediate cell wall structure (Beveridge and Graham 1991). As noted above, the decolorization step is critical to the success of the procedure.
Which came first gram-positive or negative?
Bacteria with thick cell walls keep the first (purple) stain and are called Gram positive. Thin walled bacteria cannot keep the first stain (purple) so when the second stain (red) is placed on the organisms they become red or Gram negative.
Which is the correct order for the application of reagents in the Gram stain quizlet?
List the 4 reagents used for the gram stain in the proper order? Crystal violet stains gram positive and gram negative cells. Iodine is used next as mordant. Alcohol decolorizes the gram negative cells, and lastly Safranin counterstains gram negative cells.
What is the Gram staining method quizlet?
Gram stain technique. A staining procedure used to identify bacterial cells as gram-positive or gram-negative. developed by christian gram in the 1800s. -Cells are stained with crystal violet and Gram iodine solution and washed with a decolorizer. -Safranin is applied as a counterstain.
Which step makes the Gram stain differential?
Gram's decolorizer, a mixture of ethyl alcohol and acetone, is then added. This is the differential step. Gram-positive bacteria retain the crystal violet-iodine complex while Gram-negative are decolorized.
What is the most important step in the 4 step Gram stain technique?
The thickness of the smear used in the Gram stain will affect the result of the stain. The step that is most crucial in effecting the outcome of the stain is the decolorizing step.
What is Gram staining and explain its procedure?
The procedure is based on the reaction between peptidoglycan in the cell walls of some bacteria. The Gram stain involves staining bacteria, fixing the color with a mordant, decolorizing the cells, and applying a counterstain. The primary stain (crystal violet) binds to peptidoglycan, coloring cells purple.
What are the four components of a Gram stain quizlet?
List the four reagents used for the Gram stain in the proper order.
- Crystal violet (primary stain)
- Iodine (mordant)
- Alcohol wash (decolorization)
- Safranin (counterstain)