What other information should you include when describing physical growth characteristics?

What other information should you include when describing the physical growth characteristics? Incubation temperature, time and the type of medium used—these all affect the appearance of bacterial growth. Colony size, color, and shape are three critical aspects of a description of bacterial growth.

Why are agar slants better for stock cultures?

Why are agar slants better suited than agar plates to maintain stock cultures? Slants are better suited because they can be capped, preventing the agar and culture from drying out. The cap also prevents airborne contaminates from entering the slant. Slants take up less storage space.

Why are slants preferred for the storage of bacteria?

Slanting gives the bacteria a greater surface area on which to grow in a tube. Agar slants are also useful in maintaining bacterial cultures, more so than stacks of Petri dishes. Multiple cultures are easily placed into test tube racks and stored under refrigeration.

Why can Alkaliphiles survive at high pHs when neutrophils Cannot?

Account for the inability of organisms to grow outside their pH ranges. Why, for instance are alkaliphiles able to survive at high pHs when neutrophiles cannot? The cells won't be far enough apart to grow individual colonies.

When describing the growth characteristics of a colony which of the following must be included?

Three critical aspects of a description of bacterial growth are colony size, color, and shape.

What are 5 characteristics of bacterial colony morphology?

Characteristics of a colony such as shape, edge, elevation, color and texture. When recording colony morphology, it is important to also record color, optical properties (translucence, sheen) and texture (moist, mucoid or dry). However, remember that color is often influenced by environment.

What factors can greatly impact the growth of bacteria in an environment or on a particular object?

Warmth, moisture, pH levels and oxygen levels are the four big physical and chemical factors affecting microbial growth. In most buildings, warmth and moisture are the biggest overall issues present.

When agar slants are used to observe cultivation characteristics Why is it preferable to use in inoculating needle rather than an inoculating loop?

This preview shows page 2 – 3 out of 3 pages. E. Why is it important to use a needle rather than an inoculating loop wheninoculating a motility tube? It is important to use a needle rather than an inoculating loop because the needleis used to transfer the specimen to the soft agar medium.

What is the primary use of slants and why?

Micro Lab Exercise 4

Question	Answer
What is the primary use of slants?	Long-term storage. Pure cultures.
What is the primary use of deeps?	To store anaerobic bacteria. To test oxygen requirements.
What is the primary use of broths?	To grow large amounts of bacteria.

Why are some plates placed in the candle jars which organisms would you expect to grow under these conditions?

The plates inside of the jar would reflect inhibited growth of anaerobic organisms, while aerobic organisms would be positively affected. These results will be skewed towards favouring aerobic organisms.

Which extremophiles applied in Biomining to solubilize metals from ores?

The microbial solubilization of metals is widely and successfully used in industrial processes called bioleaching of ores or biomining, to extract metals such as copper, gold, uranium, and others. This procedure is done by using chemolithoautotrophic microorganisms capable of oxidizing iron and sulfur (13, 22,30).

Why is it important to determine colony cultural characteristics?

It is important in microbiology to determine the characteristics of bacteria such as colonies, the rapidity of their development, their action upon the medium, size, shape, and appearance to identify the bacteria.

What are the 5 basic categories of colony morphology?

Based on their form, bacterial colonies are categorized as circular, irregular, filamentous, spindle, rhizoid, and concentric. Based on their margin, they are categorized as entire, lobate, undulate, filamentous, and irregular.

What factors are used in colony morphology?

Colony morphological characteristics may be viewed with the naked eye, a hand lens, a stereo (dissecting) microscope, or a colony counter (Fig. 2.3). The seven basic categories include colony size, shape, margin (edge), surface, elevation, texture, and optical properties (Fig. 2.4).

What are the 7 characteristics of bacterial colony morphology?

Colonies are described on the basis of size, shape, texture, elevation, pigmentation, and effect on growth medium.

What are 5 environmental factors that influence the growth of organisms?

Temperature, oxygen, pH, water activity, pressure, radiation, lack of nutrients…these are the primary ones.

What is the most important environmental factor influencing bacterial growth?

The phyla abundances were affected by the environmental factors to different extent, and we found pH, instead of Cu concentration, influenced UniFrac distance significantly and was identified as the most important environmental factor affecting bacterial community.

What are the precautionary measures that must be observed to ensure that the culture media are free from contamination?

How To Prevent Cell Culture Contamination

Wear gloves, lab-coats and use hoods. …
Use your hood correctly. …
Clean your incubator and water bath regularly. …
Spray EVERYTHING with ethanol or IMS. …
Minimize exposure of cells to non-sterile environments.

Mar 29, 2018

What are the 5 factors that influence microbial growth?

CHAPTER 6 – Microbial Growth

Physical factors – temp, pH, water, oxygen, pressure.
Nutrients – Cell constituents and energy sources.

What three conditions are ideal for bacteria to grow?

Bacteria can live in hotter and colder temperatures than humans, but they do best in a warm, moist, protein-rich environment that is pH neutral or slightly acidic. There are exceptions, however. Some bacteria thrive in extreme heat or cold, while others can survive under highly acidic or extremely salty conditions.

Why is biomining considered more environmental friendly than typical traditional mining techniques?

Biomining is an environmentally friendly technique compared to typical mining. Mining releases many pollutants while the only chemicals released from biomining is any metabolites or gasses that the bacteria secrete. The same concept can be used for bioremediation models.

What is biomining used for?

Biomining is the process of using microorganisms (microbes) to extract metals of economic interest from rock ores or mine waste. Biomining techniques may also be used to clean up sites that have been polluted with metals.

What aspects of colonies are important for observation of microorganisms?

Observing colony morphology is an important skill used in the microbiology laboratory to identify microorganisms. Colonies need to be well isolated from other colonies to observe the characteristic shape, size, color, surface appearance, and texture. Another important characteristic of a bacterial colony is hemolysis.

Why is it so important to determine the genus and species of an organism causing disease?

This information often can allow identification of an organism to the genus level, or can minimize the possibility that it belongs to one or another group.

What are the 4 most important environmental factors that influence bacterial growth?

What are the important environmental factors?

Air, water, climate, soil, natural vegetation and landforms are all environmental factors. By definition, the environmental factors affect everyday living, and play a key role in bringing health differences across the geographic areas.

What other factors could influence the growth rate?

Population growth rate is affected by birth rates, death rates, immigration, and emigration. If a population is given unlimited amounts of food, moisture, and oxygen, and other environmental factors, it will show exponential growth.

What are the 5 environmental factors?

What are the considerations to follow when preparing a culture media?

Five Tips for Culture Media Preparation Success

Reduced growth/recovery rates.
Atypical colonial morphology.
Inhibition of target organism.
Failure to inhibit competing flora.
Reduced shelf life of prepared medium.

Mar 21, 2018

What are the major concerns that should be taken care of to avoid contamination?

What are the major concerns that should be taken care to avoid contamination? – The best way to avoid contamination is by cleanliness. Keep the work area as clean as possible, wiping down all surfaces with a 10% commercial bleach solution or ethanol solution before stating to work.