Why is it advisable to use a positive control along with the organism’s you are testing Why is it advisable to use a negative control along with the organism’s you are testing?
why is it advisable to use a positive control along with organisms that you are testing? o make sure your conditions are correct. If your positive control gives a negative answer, then your conditions were incorrect. If your positive control gives a positive response, then your conditions are usable for testing.
Why is it necessary to use two controls rather than just one what are the specific purposes of each?
Because only one uninoculated tube is sufficient to show the green color unchanged, why is it necessary to use two controls? Be specific. They are used for color comparison. It is important to use 2 controls because the tubes show "no color change" under 2 different conditions (anaerobic and aerobic).
How would you expect the growth on the plates inside the jar to differ from the plates incubated outside the jar?
b. How would you expect the growth on the plates inside the jar to differ from the plates incubated outside the jar? –The plates inside of the jar would reflect inhibited growth of anaerobic organisms, while aerobic organisms would be positively affected. These results will be skewed towards favoring aerobic organisms.
Why might the amount or type of growth seen on the plates of two different groups that sampled the same site be different?
Why might the amount or type of growth seen one the plates of two different groups that sampled the same sight be different? The might contain diff. types of bacteria that might not grow a lot due to the nutrients and the temperature it was stored in.
Why is it necessary to have positive and negative controls in this experiment?
It is necessary to have positive and negative controls in an experiment to ensure that the results are due to the independent variable.
Why is it advisable to include an oxidase positive control organism in any test of an unknown organism?
why is it advisable to include a known oxidase positive control in any test of an unknown organism? it is always a good idea to run a positive control to ensure that a particular test system is working properly.
Were the uninoculated controls positive or negative controls and what purpose did they serve?
Were the uninoculated controls in this lab positive or negative, and what purpose did they serve? The controls were negative, and served as color comparison for experimental tubes as well as verification for media sterility.
What is the purpose of the control tubes used in this test Why is it necessary to use two?
Two uninoculated control tubes are needed to show the results of the medium in both aerobic and anaerobic environments. It is used to show that the medium remains green under both conditions, showing it is sterile and also as a color comparison.
Why are some plates placed in the candle jars which organisms would you expect to grow under these conditions?
The plates inside of the jar would reflect inhibited growth of anaerobic organisms, while aerobic organisms would be positively affected. These results will be skewed towards favouring aerobic organisms.
Did E coli grow better inside the brewer jar or outside the brewer jar explain your answer?
This can display how bacteria respond to oxygen. E. coli will not grow well in the Brewer jar.
Why is it important to have a pure culture and not a mixed culture when performing biochemical tests?
These pure cultures are important to microbiologists as they allow for the study of one species without the worry of contamination from other organisms.
Why is it so important to determine the genus and species of an organism causing disease?
This information often can allow identification of an organism to the genus level, or can minimize the possibility that it belongs to one or another group.
Why is it important to include a negative control well on the Biolog plate?
Why do you need to assay positive and negative control samples as well as your own experimental samples? Controls are needed to make sure the experiment worked. if there are no positive controls and the sample is negative, we can't know if the sample was truly negative or if the assay process didn't work.
Why do you need to assay positive and negative control samples as well as your experimental samples?
The importance of including ELISA controls, both positive and negative, in your immunoassay helps to verify that the assay was run properly and everything is performing accurately.
Why is it advisable to perform this test on a known catalase positive organism along with the organism you are testing?
Why is it advisable to perform this test on a known catalase positive organism along with the organism you are checking? The known catalase positive organism serves as a positive control to ensure that the peroxide reagent is good.
Why is the oxidase test important for differentiating bacteria?
The oxidase test is used to determine if an organism possesses the cytochrome oxidase enzyme. The test is used as an aid for the differentiation of Neisseria, Moraxella, Campylobacter and Pasteurella species (oxidase positive). It is also used to differentiate pseudomonads from related species.
Why is a negative control so important and why is it important to conduct multiple trials in an experiment?
Negative controls are important in experimental design. The negative control makes sure that there isn't anything strange going on that might be mistaken for a result.
What was the purpose of the control tubes?
The major role of control tube is to reduce the variability due to unwanted/unknown factors of the experiment.
Why do microorganisms differ in their requirements for growth?
Even when nutrients are available and the temperature is right, many other environmental factors can influence the growth of microbes. These include acidity, availability of water, and atmospheric pressure. Each microbe prefers a range of properties for multiple features of the environment.
What would you need to determine whether bacteria growing on a petri plate from the brewer jar are anaerobes?
requires presence of oxygen.
Which of the organisms would not have grown if placed in the anaerobic jar?
Clostridium, a known obligate anaerobe, does not grow in the GasPak jar. What might be the reason for this lack of growth? The chemical reaction failed to remove all of the oxygen in the jar.
Why is it important to separate mixed communities of microbes into individual pure cultures of each different species?
Microbes grow more slowly in mixed cultures, so separating them out will help their growth rate.
Why is it necessary to isolate individual colonies from a mixed growth?
Why is necessary to isolate individual colonies from a mixed growth? Ans: Every individual colony in a mixed growth might present different bacteria. We isolate them to help us effectively to determine on their differences. 7.
Why might it be difficult to decide whether an organism should be classified as a microbe or not?
Why might it be difficult to decide whether an organism should be classified as a microbe or not? – Some multicellular animals are microscopic. You isolate a human pathogen and note that it is a cellular organism that contains a nucleus.
Why is it necessary to determine the cultural characteristics of microorganisms?
The cultural characteristics of microorganisms will helps in differentiating microorganisms on basis of their physical appearances.
Why is it not necessary to include a negative control?
why is it not necessary to include a negative control for the endospore stain procedure? because an endospore stain is a differential type of staining procedure. it allows us to see both spores and vegetative cells. therefore, including a separate negative control consisting of only vegetative cells isn't necessary.
Why is it important to include negative and positive controls when performing a test?
Negative controls are particular samples included in the experiment that are treated the same as all the others but are not expected to change from any variable in the experiment. The positive control sample will show an expected result, helping the scientist understand that the experiment was performed properly.
Why is it important to always have a positive and negative control when performing these tests?
The importance of including ELISA controls, both positive and negative, in your immunoassay helps to verify that the assay was run properly and everything is performing accurately.
Why is it advisable to include a known oxidase positive control in any test of an unknown organism?
why is it advisable to include a known oxidase positive control in any test of an unknown organism? it is always a good idea to run a positive control to ensure that a particular test system is working properly.
Why does catalase test should not be performed on bacterial colonies taken from blood agar?
Note: The catalase test should not be performed on colonies taken from media containing whole red blood cells because they contain catalase and could therefore give a false positive result.