What features do the cells you observed have in common?
Some features in common: nuclei cytoplasm and cell membranes. Some different features: cell walls chloroplasts and cilia/flagella.
Why wouldn’t it be advisable to compare growth of the organisms on each plate to each other?
why wouldn't it be advisable to compare growth of the organisms on each plate to each other? there are at least two answers to this question. You do not know how the inhibitors may have affected the growth of the different organisms. So you can't compare growth on selective media.
Why would an organism that possesses the citrate lyase enzyme not test positively on Simmons citrate agar Would this be a false negative result?
Citrate lyase, which catalyzes the conversion of citrate into oxaloacetate and acetate, is associated with how citrate is used, not how it gets into the cell. So, an organism could be citrate-negative (it doesn't make citrate permease), but still be citrate lyase-positive.
What ingredients are in Columbia CNA supplies carbon?
Which ingredient(s) in Columbia CNA plus 5% sheep blood agar supply(ies) carbon? Casein, animal tissue, yeast extract, beef extract, and corn starch can all supply carbon.
What structures are common to all cells?
No matter which type of cell we are considering, all cells have certain features in common, such as a cell membrane, DNA and RNA, cytoplasm, and ribosomes. Eukaryotic cells have a great variety of organelles and structures.
What are the similarities and differences of prokaryotic and eukaryotic cells?
Like a prokaryotic cell, a eukaryotic cell has a plasma membrane, cytoplasm, and ribosomes, but a eukaryotic cell is typically larger than a prokaryotic cell, has a true nucleus (meaning its DNA is surrounded by a membrane), and has other membrane-bound organelles that allow for compartmentalization of functions.
What does the enzyme Casease have in common with amylase How are they different?
What does the enzyme casease have in common with amylase? They are both exoenzymes. Casease hydrolyzes casein while amylase hydrolyzes starch. Some microbiologists recommend incubating this medium at 37C, along with an uninoculated control, and then transferring all tubes to the refrigerator prior to reading them.
Would it likely make an important difference if you began Streak 2 near S aureus and streaked across the plate in the other direction Why?
It wouldn't make an important difference if you streaked the other way because the hemolysis would still be greater near the S. aureus, so it would still be an arrowhead shape.
What is the purpose of the Simmons citrate test what specifically will it reveal about your sample?
Simmons Citrate agar is used to test an organism's ability to utilize citrate as a source of energy. Ammonium Dihydrogen Phosphate is the sole source of nitrogen.
What is the purpose of citrate utilization test what are the products of this reaction and how are these products detected?
Citrate testing is used to determine the ability of the bacteria to use sodium citrate as the only source of carbon and inorganic ammonium hydrogen phosphate (NH4H2PO4) as a source of nitrogen. The citrate utilization test is possible only if the organisms are capable of fermenting citrate.
What are the roles of colistin and nalidixic acid in CNA?
In your own words, what ar the roles of colistin and nalidixic acid in CNA and how does each work? Both are antibiotics that affect gram – negative cells. Colistin disrupts membrane integrity and nalidixic acid affects DNA replication.
Is Columbia CNA a defined or undefined medium because of what ingredients?
Is Columbia CNA agar a defined or an indefine medium? Provide the reasoning behind your choice and explain why this formulation is desirable. It is undefined due to the casein, animal tissue, yeast extract, beef extract, and corn starch in the medium.
What are common cells?
List of distinct cell types in the adult human body
| Cell type | % cell count |
|---|---|
| Erythrocytes (red blood cells) | 84.0 |
| Platelets | 4.9 |
| Bone marrow cells | 2.5 |
| Vascular endothelial cells | 2.1 |
What is common in every cell?
Parts common to all cells are the plasma membrane, the cytoplasm, ribosomes, and genetic material.
What are the differences and similarities?
A similarity is a sameness or alikeness. When you are comparing two things — physical objects, ideas, or experiences — you often look at their similarities and their differences. Difference is the opposite of similarity. Both squares and rectangles have four sides, that is a similarity between them.
What are the difference between the two types of cell?
There are only two main types of cells: prokaryotic and eukaryotic. Prokaryotic cells lack a nucleus and other membrane-bound organelles. Eukaryotic cells have a nucleus and other membrane-bound organelles. This allows these cells to have complex functions.
What does Casease breakdown?
Casease allows the organisms that produce it to break down casein into smaller polypeptides, peptides, and amino acids that can cross the cell membrane and be utilized by the organism.
Why do some protocols suggest re incubating organisms producing methyl red negative results for an additional 2 3 days?
Some microbiologists recommend reincubating organisms producing methyl red-negative results for an additional 2 to 3 days. Why do you think this is done? It gives the organism enough time to lower pH in order to ensure you don't produce a false negative result.
Which of the following is utilized to distinguish between members of the family Enterobacteriaceae and differentiate them from other gram negative rods?
virtually all Enterobacteriaceae perform a one-step reduction of nitrate to nitrite. The nitrate test differentiates them from Gram-negative rods that either do not reduce nitrate or reduce it beyond nitrite to N2 or other compounds.
Why is it a good idea to run a positive control in both the slide and tube test?
Why is it a good idea to run a positive control in both the slide and tube test? b. It provides a baseline so you should not witness a change.
What is the purpose of Simmons citrate agar?
Purpose. This medium is used to aid in the differentiation of various genera in the Enterobacteriaceae. Bacteria that can utilize citrate as the sole carbon source and inorganic ammonium salt as the sole source of nitrogen grow on this medium.
What is the citrate test testing the organism for quizlet?
-the purpose of the citrate utilization test is to identify intestinal bacteria that are capable of using citrate as their sole carbon source. -this ability depends on the production of an enzyme called citrate permease. -facilitates the transport of citrate from the medium into the cytoplasm of the bacterium.
Is Columbia a CNA differential?
BD BBL Columbia CNA Agar with 5% Sheep Blood is a selective and differential medium used for the isolation and differentiation of gram-positive microorganisms from clinical and nonclinical materials.
Is CNA a differential?
The CNA-blood agar, like the TSA-blood agar is differential. The differential material in both is defibrinated red blood cells.
What is the application of Columbia CNA?
Columbia CNA Agar is used with blood for the selective isolation of Gram-positive cocci in a laboratory setting. Columbia CNA Agar is not intended for use in the diagnosis of disease or other conditions in humans.
What is the differential agent in CNA?
The CNA-blood agar, like the TSA-blood agar is differential. The differential material in both is defibrinated red blood cells. Bacteria that can metabolize whole RBC's do so by producing the enzyme hemolysin.
What features do all cells have?
All cells have a cell membrane, cytoplasm, and DNA. cells differ in how their genetic information is contained. Understanding how cells function makes it easier to learn how organisms function.
What are the differences between the two types of cells?
There are only two main types of cells: prokaryotic and eukaryotic. Prokaryotic cells lack a nucleus and other membrane-bound organelles. Eukaryotic cells have a nucleus and other membrane-bound organelles. This allows these cells to have complex functions.
How are we the same and different from each other?
We are mammals, primates, and intelligent vertebrates, who are born, grow, change, and die. We are also the same because we are all different. We have thoughts, ideas, beliefs, talents, and dreams, but how we think and act makes us who we are. Children often feel that they need to be the same as everyone else.
How do you find similarities and differences?
Venn diagrams, matrices, and T-charts are all powerful tools to help students compare. In the primary grades, students can compare two objects, people, or places based on firsthand experience or experimentation. Students can also begin to compare and contrast after listening to a story.